Fig. 4

Heatmap visualisations comparing the enrichment values for mutations of aminoglycoside 3'-phosphotransferase. A A heatmap representing the entire mutational space of aminoglycoside 3'-phosphotransferase. B A heatmap representing only the mutations of aminoglycoside 3'-phosphotransferase that were predicted by the SSP. The X-axis represents the antibiotics and their tested concentrations, while the Y-axis represents the relevant mutations of aminoglycoside 3'-phosphotransferase. Details of antibiotic concentrations and individual enrichment values can be found in SI 1. Each rectangle on the plot indicates the enrichment value for a mutation when exposed to the effects of the specific antibiotic concentration. The Viridis colour map is used to maintain perceptual uniformity. A value of 1 (dark blue) represents no change in enrichment from the wild type, anything below 1 (purple) represents a negative effect on enrichment, while anything above 1 (light blue to yellow) represents a positive enriching effect of the mutation. This figure contrasts the effects of random mutations on the activity of aminoglycoside 3'-phosphotransferase, against the effect of SSP suggested mutations for the same protein. The perceptual increase in ‘brightness’ of B over A illustrates an increase in the positive impact of mutations on the activity of aminoglycoside 3'-phosphotransferase